Journal: Scientific Reports

Article Title: Single-cell RNA sequencing reveals that a high salt diet increases IL6R-JAK1-STAT3 gene signaling pathway activity in intestinal B cells and Tfh cells of salt-sensitive hypertension animal model

doi: 10.1038/s41598-025-11100-z

Figure Lengend Snippet: HSD intake increases B cell population in the SILPs of SS rats. ( A , C ) UMAP plots display clusters of CD45+ cells in the SILPs. ( A ) Annotations indicate cell types based on gene expression. ( B ) Dot plot shows representative gene expression for each cell type. ( C ) Red dots represent the NSD group, and blue dots represent the HSD group. ( D ) Bar plot shows the proportion of immune cells in the SILPs ( n = 4/group). # p = 0.05 by t-test. ( E ) Bar plots represent the distribution of B cells (CD45+ CD79a+) in the SILPs ( n = 4/group) using flow cytometry. * p < 0.05 in a t-test.

Article Snippet: Antibodies used for staining B cells and Tfh cells in this study as follows : CD45-PE/Cyanine7 (Biolgend, San Diego, CA, USA, 202214), CD79a-PE (Novus Biologicals, Centennial, CO, USA, NBP2-54469PE), CD3-BV421 (BD, Franklin Lakes, NJ, USA, 563948), CD4-APC Cy7 (BD, 565432), CXCR5-unconjugated (Invitrogen, Waltham, Massachusetts, USA, MA5-41258), and secondary antibody-FITC (Invitrogen, A-11034).

Techniques: Gene Expression, Flow Cytometry

Journal: Journal of immunological methods

Article Title: A high-throughput multiplex assay to characterize flavivirus-specific immunoglobulins.

doi: 10.1016/j.jim.2020.112874

Figure Lengend Snippet: Fig. 3: Selection of sample dilution. All samples were run in 3 dilutions. The signal resolution was calculated by dividing the MFI signal of the sample to that of the corresponding naive sample (plasma or serum). The graph represents the distributions of the signal-to-noise-ratio (S/N) for each dilution stratified by detection antibody (IgA, total IgG and IgM) and virus (DENV 1-4 and ZIKV). Dotted vertical lines represent the median.

Article Snippet: After three washes with 50μl of wash buffer (PBS 1X,20mM TrisHCl pH7.5, 0.05% Tween), antibody responses were detected with 20μl of phycoerythrin (PE)-labeled secondary antibodies specific for IgA, IgA1, IgA2, IgG1, IgG2, IgG3, IgG4, total IgG and IgM (Southern Biotech; 2050-09, 9140-09, 9130-09, 9040-09, 9054-09, 9070-09, 9210-009, 9200-09 and 9020-09) diluted in assay buffer at a concentration of 3μg/ml for 30 minutes.

Techniques: Selection, Clinical Proteomics, Virus

Journal: Journal of immunological methods

Article Title: A high-throughput multiplex assay to characterize flavivirus-specific immunoglobulins.

doi: 10.1016/j.jim.2020.112874

Figure Lengend Snippet: Fig. 4: Large range of MFI signals. The MFI signal measured for each sample is represented for all the detection antibodies (IgA, IgA1, IgA2, total IgG, IgG1-4 and IgM) and antigens tested (colors represent the different antigens). The black dotted line corresponds to 300 MFI (background signal).

Article Snippet: After three washes with 50μl of wash buffer (PBS 1X,20mM TrisHCl pH7.5, 0.05% Tween), antibody responses were detected with 20μl of phycoerythrin (PE)-labeled secondary antibodies specific for IgA, IgA1, IgA2, IgG1, IgG2, IgG3, IgG4, total IgG and IgM (Southern Biotech; 2050-09, 9140-09, 9130-09, 9040-09, 9054-09, 9070-09, 9210-009, 9200-09 and 9020-09) diluted in assay buffer at a concentration of 3μg/ml for 30 minutes.

Techniques:

Journal: Journal of immunological methods

Article Title: A high-throughput multiplex assay to characterize flavivirus-specific immunoglobulins.

doi: 10.1016/j.jim.2020.112874

Figure Lengend Snippet: Fig. 7: Responses across ZIKV infection stages. This graph represents the distribution of signal-to- noise ratios of IgA, total IgG and IgM responses against ZIKV antigens (summary values pooling all antigens) at each stage of the infection: acute (< 30 days post-onset of symptoms (DPO), in purple), convalescent (30 < 90 DPO, in blue) and late convalescent (> 90 DPO, in light blue). The dashed black and red lines represent a S/N of 1 and 3, respectively. Wilcoxon test; ns: p > 0.05; *: p <= 0.05; **: p <= 0.01; ***: p <= 0.001; ****: p <= 0.0001.

Article Snippet: After three washes with 50μl of wash buffer (PBS 1X,20mM TrisHCl pH7.5, 0.05% Tween), antibody responses were detected with 20μl of phycoerythrin (PE)-labeled secondary antibodies specific for IgA, IgA1, IgA2, IgG1, IgG2, IgG3, IgG4, total IgG and IgM (Southern Biotech; 2050-09, 9140-09, 9130-09, 9040-09, 9054-09, 9070-09, 9210-009, 9200-09 and 9020-09) diluted in assay buffer at a concentration of 3μg/ml for 30 minutes.

Techniques: Infection